In vitro plantlet regeneration from cotyledon explants of Capsicum annuum L
1 Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Bangladesh Atomic Energy Commission, G.P.O. Box No. 3787, Dhaka-1000, Bangladesh.
2 United Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Saiwaicho, Fuchu-shi, Tokyo, Japan.
3 Department of Biotechnology and Genetic Engineering, University of Development Alternative (UODA), Dhanmondi, Dhaka, Bangladesh.
4 Scientific Information Unit, Atomic Energy Research Establishment, Bangladesh Atomic Energy Commission, G.P.O. Box No. 3787, Dhaka-1000, Bangladesh.
Review
International Journal of Scientific Research Updates, 2022, 04(02), 195–200.
Article DOI: 10.53430/ijsru.2022.4.2.0181
Publication history:
Received on 14 October 2022; revised on 27 November 2022; accepted on 30 November 2022
Abstract:
An efficient In vitro plant regeneration was established in Capsicum annuum L. from cotyledon explants. The best performance i.e., 80% of the explants responded for multiple shoot bud proliferation on MS medium containing 10 mg/l Zeatin. The maximum number of buds per explants was 50.28 ± 2.69 and the bud length of 0.5 ± 0.22 cm was obtained in this medium after 30 days. The average number of 80.32 ± 0.16 multiple shoot bud and the average shoot bud length of 1.0 ± 0.19 cm was attained on the same medium after 90 days. These shoot buds on transfer to MS + 10 mg/l Zeatin + 2.0 mg/l GA3 yielded 6.3 ± 0.35 number of multiple shoots with an average shoot length of 4.6 ± 0.62 cm after 30 days. Shoots rooted well (80% of induced shoots) on half strength MS medium supplemented with 0.5 mg/l IBA. The average number of roots per shoot was 8.2 ± 0.79 and the average root length of 4.5 ± 0.58 cm was recorded. About 80% of In vitro derived plantlets were survived under open field conditions.
Keywords:
In-vitro; Capsicum; Cotyledon; Regeneration; Zeatin
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