Synergistic Supplementation of Lemuru Fish Oil and Corn Oil Enhances Sperm Quality in Kampung Chickens
1 Department of Animal Science, Faculty of Animal Husbandry, University of Nahdlatul Wathan Mataram, Mataram, Indonesia.
2 Department of Agriculture, Faculty of Agriculture, University of Muara Bungo, Jambi, Indonesia.
Research Article
International Journal of Scientific Research Updates, 2025, 10(02), 012-016.
Article DOI: 10.53430/ijsru.2025.10.2.0044
Publication history:
Received on 09 September 2025; revised on 19 October 2025; accepted on 22 October 2025
Abstract:
This study investigated the effects of dietary supplementation with lemuru fish oil (Sardinella longiceps, LFO), corn oil (Zea mays L., CO), and their combination on spermatozoa quality in kampung chickens. Sixteen adult males were assigned to four treatment diets: R-0 (control), R-1 (10% LFO), R-2 (5% LFO + 5% CO), and R-3 (10% CO), each with four replicates. Diets were formulated to be iso-nitrogenous and iso-caloric following current poultry nutrient standards. Semen samples were collected using the abdominal massage method and analyzed for sperm motility and concentration. Results showed that supplementation with LFO and CO significantly (P < 0.05) improved both sperm motility and concentration compared with the control. The combination of 5% LFO and 5% CO (R-2) produced the highest motility (88.34%) and concentration (3.07 × 10⁹/mL), indicating a synergistic effect between omega-3 and omega-6 fatty acids. The balanced fatty acid profile enhanced lipid metabolism, steroid hormone synthesis, and sperm membrane integrity, leading to better reproductive performance. It can be concluded that the combined supplementation of 5% LFO and 5% CO provides optimal improvement in kampung chicken spermatozoa quality.
Keywords:
Kampung Chicken; Lemuru Fish Oil; Corn Oil; Sperm Quality
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