Histo-pathology of the Vibrio alginolyticus bacteria on tiger grouper (Epinephelus fuscoguttatus Forskal)
Aquaculture Department, Faculty of Fisheries and Marine, Riau University, Pekanbaru, Indonesia.
Research Article
International Journal of Scientific Research Updates, 2022, 04(01), 096–102.
Article DOI: 10.53430/ijsru.2022.4.1.0094
Publication history:
Received on 20 June 2022; revised on 26 July 2022; accepted on 28 July 2022
Abstract:
The purpose of this study was to determine histo-pathology of bacterium V. alginolyticus against several tiger grouper fish organs. The pathogenicity test was carried out by injecting intramuscularly (IM) 0.1 ml of V. alginolyticus bacterial suspension with bacterial density of 107, 108, 109 cells/ml/fish and injected with 0.1 ml of PBS solution (phospate buffer solution) as a control. Results showed that bacterium V. alginolyticus causes degeneration, damage and structural changes in the tissues of gill organs, kidneys, liver, and brain of tiger grouper fish. Pathogenicity challenge tests also prove that this bacterium is a true pathogen for test animals. The degree of density of the suspension of pathogenic cells affects mortality and incubation period. The bacterial density of 107 cells/ml/fish has caused death of 33.33%, density of 108 cells/ml/fish causes’ death of 66.67%, and density of 109 cells/ml/fish causes death of 73.33%, while without bacteria (control) there is no death.
Keywords:
Histo-Pathology; Pathogecity; Vibrio alginolyticus; Tiger grouper
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