Effect of aqueous leaf extract of Macaranga barterion selected haematological and biochemical indices of carrageenan -induced paw edema in Wistar albino rats
Department of Biochemistry, University of Port Harcourt, Choba, Rivers State, Nigeria.
Research Article
International Journal of Scientific Research Updates, 2022, 04(02), 092–101.
Article DOI: 10.53430/ijsru.2022.4.2.0074
Publication history:
Received on 04 August 2022; revised on 30September 2022; accepted on 03 October 2022
Abstract:
This research was designed to determine the anti- inflammatory and quantitative characterization of phenolic compounds in aqueous leaf extract of Macaranga barteri on carrageenan induced paw edema in wistar rats. Phenolic compound was characterize using GC-MS. Twenty wistar rats of both sexes weighing between 120-140g were grouped into 5 with four (4) rats per group. Group 1 served as the normal control which received distilled water, group 2 (negative control) rats received 1% carrageenan only, groups 3 and 4 were treated with aqueous leaf extract of M. barteri, group 5 was treated with diclofenac, 30 minutes after receiving 1% carrageenan, the changes in paw sizes were observed hourly for 3 hours. Rats were sacrificed and blood samples collected for haematological and biochemical analyses. Phenolic compounds detected were 2-nitro phenol (3.29 ppm) and 4-chloro-3-methyl-phenol (5.09ppm). Significant reduction in rat paws oedema was observed when rats in group 4 were compared with group 2 at 3hours with percentage oedema inhibition of 46.91. Mean ESR of rats in groups 2, 3 and 5 significantly increased and group 4 none significantly reduced when compared to control. Non-significant differences in mean WBC, neutrophil and lymphocyte counts, significant reduction of mean eosinophil counts in groups 2 and 3 and non-significant increase in group 4 when compared to control. Significant increase in mean CRP concentration was observed when group 2 was compared to control group and a significant reduction in group 4 treated with 500mg/kgb.wt. When compared to group 2. Mean concentrations of Il-6 and prostaglandin significantly increased in all the groups when compared to control. Aqueous leaf extract of M. barteri demonstrated near average percentage paw oedema inhibition and mild anti-inflammatory potential at 500mg/kg b.wt.
Keywords:
Anti- Inflammatory; Carrageenan; Phenolic; Haematological; Biochemical; Prostaglandin
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