Antitumor activity of protein fraction of Jatropha curcas In DMBA and UV B induced skin tumors in mice: Expression of Bcl-2
Department of Pharmacy, Faculty of Health Sciences, Jenderal Soedirman University, Purwokerto, Central Java, Indonesia.
Research Article
International Journal of Scientific Research Updates, 2024, 08(01), 076–081.
Article DOI: 10.53430/ijsru.2024.8.1.0049
Publication history:
Received on 29 June 2024; revised on 12 August 2024; accepted on 15 August 2024
Abstract:
UV radiation and 7,12-dimethylbenz[α]anthracene (DMBA) induced skin tumors can be seen from the decreased Bcl-2 as a suppressor gene regulating programmed cell death and apoptosis. This study aims to determine the antitumor activity of protein fractions from Jatropha curcas leaves against skin tumor cells through the expression of Bcl-2 protein in mice-induced DMBA and UVB. This study used 40 mice divided into 4 groups. Group 1 was the control group without treatment. Group 2 was induced 0.2% DMBA (200μl/mice) and UVB (5x/week) for 8 weeks. Groups 3 and 4 were induced 0.2% DMBA (200μl/mice) and UVB (5x/week) for 8 weeks of continued treatment with DMSO, and the protein fraction dose of 2 mg/0,1 ml for 4 weeks, respectively. Immunohistochemistry examination from the tissue skin of mice was performed by using Bcl-2 monoclonal antibody on week 8 (after induced UVB) and on week 12 (after treatment). The results showed that Bcl-2 expression was significantly different in group 2 (induced DMBA and UVB) and group 4 (protein fraction) (p<0,05). Expression of Bcl-2 was found to be lowest in the group with treatment by protein fraction dose of 2 mg/0,1 ml. The highest expression was found in the group with induced DMBA and UVB. Decreased expression of Bcl-2 is a sign of apoptosis, so the protein Jatropha curcas has antitumor through induction of apoptosis.
Keywords:
Jatropha curcas; Skin tumor; Bcl-2; Apoptosis
Full text article in PDF:
Copyright information:
Copyright © 2024 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0