A simple oxidative coupling-based spectrophotometric method for the determination of Mesalazine in pure form and pharmaceutical formulations
Department of Chemistry, College of Science, University of Kirkuk, Kirkuk, Iraq.
Research Article
International Journal of Chemical and Pharmaceutical Research Updates, 2026, 06(02), 034-044.
Article DOI: 10.53430/ijcpru.2026.6.2.0039
Publication history:
Received on 17 May 2026; revised on 25 June 2026; accepted on 27 June 2026
Abstract:
A straightforward, rapid, precise, and sensitive spectrophotometric technique has been established for the quantification of mesalazine (MES) in both its pure form and within pharmaceutical tablet formulations. This method relies on the oxidative coupling reaction that occurs between mesalazine and the metol reagent, facilitated by potassium periodate (KIO₄) acting as the oxidizing agent and sodium hydroxide (NaOH) serving as the alkaline medium, leading to the formation of a stable purple-hued chromogen exhibiting maximum absorbance at 628 nm. The experimental conditions affecting color development were optimized, including reagent volume, oxidant volume, alkaline medium, order of addition, temperature, and reaction time. Beer's law was obeyed over the conc. range of 3.0-20.0 µg mL⁻¹ with the regression equation A = 0.0386C + 0.1432 and a coefficient of determination (R²) of 0.9991. The method showed good accuracy and precision, with recoveries of 98.87-101.20% for the pure drug and 98.13-101.00% for tablet analysis. The D.L was 0.1759 µg mL⁻¹ and the limit of quantitation was 0.5863 µg mL⁻¹. The suggested approach was worked implemented to AwaSalazin® 500 mg tablets with no significant interference from common excipients, indicating its suitability for routine quality-control analysis.
Keywords:
Mesalazine; Metol; Potassium Periodate; Oxidative Coupling Reaction; Spectrophotometry; Pharmaceutical Preparations
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